Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. For instance, this amine can be coupled with EDC-activated carboxylic groups. With this bright and photostable reagent, many different methods of fluorescence detection is available and colorful fluorophore can also be easily detected in small quantities by naked human eye. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
