Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3’ end.Features of TaqMan Gene Expression assays include:? Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required? Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay? Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets? Accurate—identify small fold-changes with high accuracy of quantitation? Extensive content—Over 1.8 million predesigned assays available for over 25 different species? Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology? Fast delivery—your assays shipped in as little as 1–2 days (see below)
